RKO-EGFP cell line

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RKO-EGFP Order

Catalog#

YC-C002-EGFP-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

EGFP Stable Cell Lines

cell name

RKO-EGFP

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Reporter gene

EGFP

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Accession

CVCL_C8W5(Cellosaurus)

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:RKO			STR Info (Cell bank) Cell Line:p53R [JHU-56]
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11	13
D13S317	8	11		8	11
D7S820	8	10		8	10
D16S539	13	13		12	13
VWA	16	22		16	22
TH01	6	10		6	10
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	8	10		8	10
D12S391	15	19	20.0
FGA	20	21	23.0
D2S1338	16	16
D21S11	27	29	30.0
D18S51	11	12
D8S1179	9	13	14.0
D3S1358	16	19
D6S1043	14	19
PENTAE	11	13
D19S433	14	14
PENTAD	10	11
D1S1656	13	14	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the RKO cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a RKO-EGFP cell line that can maintain long-term, stable and high expression.

RKO-EGFP

Catalog#

YC-C002-EGFP-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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RKO-EGFP cell line

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